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Test Code TSPT T-SPOT.TB

Important Note

  • T-Spot specimens cannot be held overnight.
  • Routine Collection Monday through Thursday.
  • Specimens collected on Friday MUST be received at HMC Laboratory by 3:00 PM

Useful For

T-Spot is a highly sentitive blood test used to screen for TB. (Mycobacterium tuberculosis). The sensitivity and specificity of the test exceeds 95%. This test is appropriate for high risk groups as well as screening. It is unaffected by the BCG vaccination.

 

Special Instructions

 

 

Specimen Requirements

  • 9 mL Vacuette® green top tube (Lithium heparin).             
  •     
  • Fill tube to line. Invert 8-10 times.    
  • Maintain upright position
  • Note: if patient is immunocompromised two tubes should be collected to assure a sufficient quantity of WBC's for testing.

Specimen Type

Whole blood.

Specimen Transport Temperature

Temperature
Ambient 15-25°C

Specimen Stability

Temperature Time Frame
Ambient 15-25°C 32 hours

 

Reject Specimen Due To

Refrigrated, frozen and centrifuged specimens will be rejected.

 

Day(s) Test Set up

Monday through Friday

 

Reference Values

 

Result Interpretation
≤ 4 spots Negative
≥ 8 spots Positive
5-7 spots Borderline*
Invalid Invalid*
   
* Repeat testing recommended

 

Methodology

IGRA: Interferon Gamma Release Assay. A purified volume of WBC’s is stimulated with TB specific antigens. The cells release interferon-gamma which is bound and measured.

 

CPT Code

86481

Loinc Code

71773-6