Test Code RFLXP Antinuclear Antibodies, HEp-2, Reflex Panel (Non-Orderable)

Important Note

This test is not orderable. Confirmatory testing is ordered by Mayo Clinical Laboratories when the ANA Ab HEp-2 (ANAR) results identify a positive homogenous, speckled, or dense fine speckled pattern.

Reporting Name

ANA Ab HEp-2 Reflex Panel

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

Confirmatory testing after a positive homogenous, speckled, or dense fine speckled pattern identified in the human epithelial type 2 antinuclear antibody indirect immunofluorescence assay

Profile Information

Test ID	Reporting Name	Available Separately	Always Performed
ADNA1	dsDNA Ab, IgG, S	Yes	Yes
RNP	RNP Ab, IgG, S	Yes	Yes
SCL70	Scl 70 Ab, IgG, S	Yes	Yes
SM	Sm Ab, IgG, S	Yes	Yes
SSA	SS-A/Ro Ab, IgG, S	Yes	Yes
SSB	SS-B/La Ab, IgG, S	Yes	Yes
IM_04	Antinuclear Ab,HEp-2,reflex Comment	No	No

Day(s) Performed

Monday through Saturday

Report Available

3 to 4 days

Reference Values

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

DOUBLE-STRANDED DNA (dsDNA) ANTIBODIES, IGG, SERUM

<100 IU/mL (negative)

≥100 IU/mL (positive)

Negative is considered normal.

Reference values apply to all ages.

RNP ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

SCL 70 ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

SMITH (Sm) ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

SS-A/RO ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

SS-B/LA ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Specimen Minimum Volume

Serum: 0.5 mL

Specimen Required

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Specimen Stability Information

Specimen Type	Temperature	Time
Serum	Refrigerated (preferred)	21 days
	Frozen	21 days

Specimen Type

Serum

Method Name

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

ADNA1: Enzyme-Linked Immunosorbent Assay (ELISA)

RNP, SCL70, SM, SSA, SSB: Multiplex Flow Immunoassay

IM_04: Technical Interpretation

Test Classification

Not Applicable

CPT Code Information

86225

86235 x5

LOINC Code Information

Test ID	Test Order Name	Order LOINC Value
RFLXP	ANA Ab HEp-2 Reflex Panel	97564-9

Result ID	Test Result Name	Result LOINC Value
ADNA1	dsDNA Ab, IgG, S	33799-8
IM_04	Antinuclear Ab,HEp-2,reflex Comment	77202-0
RNP	RNP Ab, IgG, S	29958-6
SCL70	Scl 70 Ab, IgG, S	47322-3
SM	Sm Ab, IgG, S	18323-6
SSA	SS-A/Ro Ab, IgG, S	33610-7
SSB	SS-B/La Ab, IgG, S	33613-1