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Test Code QNTBR Culture, Quantitative Bronch with Gram Stain

Performing Laboratory

Hunterdon Medical Center Laboratory

Special Instructions

Quantitative cultures of bronchial alveolar lavage (BAL) and protected bronchial brush (PSB) specimens can be useful in the diagnosis of ventilator-associated pneumonia, pneumonia in immunocompromised patients and cystic fibrosis patients, acute exacerbation in COPD patients and in community acquired pneumonia.

Specimen Required

Sterile container.

1. BAL: 40-80ml of fluid.

2. PSB: place brush in 1.0 ml sterile non-bacteriostatic saline.

Specimen Type

1. Bronchial alveolar lavage (BAL)

2. Protected specimen brush (PSB)

Specimen Transport Temperature

Ambient:  Deliver within 2 hours.

If delivery is delayed: Refrigerate 2-8°C.

Specimen Stability Information

24 hours.

Reject Due To

Bronchial wash specimen is not acceptable for this procedure.

Day(s) Performed

Monday through Sunday

Reference Values

Normal flora.

Method Name

Conventional Culture and Biochemical Identification methods. A calibrated loop is used for quantitation.