Specimen Required

Specimen Type: Platelet-poor plasma

Patient Preparation:

1. Patient should not be receiving anticoagulant treatment (eg, warfarin, heparin). Warfarin will lower protein S. If not possible for medical reasons, note on request.

 a. If medically feasible, for 4 to 6 hours before specimen collection, do not administer intravenous heparin.

 b. If medically feasible, for 10 to 14 days before specimen collection, do not administer subcutaneous heparin or warfarin.

2. Patient should not be receiving fibrinolytic agents (streptokinase, urokinase, tissue plasminogen activator [tPA]).

3. It is recommended that specimens be collected pretransfusion. If patient has been transfused, a specimen should not be collected for 48 hours.

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vials (Polypropylene preferred)

Specimen Volume: 1 mL Platelet-poor plasma in 2 plastic vials, each containing 0.5 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot 0.5 mL of plasma into 2 plastic vials, leaving 0.25 mL in the bottom of centrifuged vial.

4. Immediately freeze plasma (no longer than 4 hours after collection) at -20° C or, ideally at -40° C or below.

5. Send specimens in the same shipping container.

Additional Information: A double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.