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Test Code METS Methadone and Metabolites, Serum

Reporting Name

Methadone and Metabolite, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Compliance monitoring of methadone

 

Assessment of methadone toxicity

Hunterdon Medical Center Note:

Clearance half life for serum: Average 30 hours.


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.0 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Type

Serum Red

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Day(s) Performed

Tuesday, Thursday

Reference Values

Methadone:

Therapeutic: 100-400 ng/mL

Toxic: >2000 ng/mL

 

EDDP:

Not established

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Methadone: 10 ng/mL

EDDP: 5.0 ng/mL

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MDNS Methadone and Metabolite, S 96602-8

 

Result ID Test Result Name Result LOINC Value
36309 Methadone 3772-1
36310 EDDP 60071-8

Report Available

3 to 5 days

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.