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Test Code CXFUN Culture, Fungal, Other Source with Fungal Stain

Performing Laboratory

Hunterdon Medical Center Laboratory

Specimen Required

Sterile container appropriate for specimen collected.

Specimen Type

Abscess/Wound drainage:

Aspirate and transport in capped syringe, without needle, or submit fluid in sterile container. Specimens collected on swabs are not optimal, but will be processed if submitted in culture transport medium to prevent drying. Submit separate swab system for each culture type requested, to provide sufficient material.

Body fluids:

Aspirate and transport in capped syringe, without needle, or submit in sterile container.

Catheter:

Place 5cm of the distal end, collected aseptically, into a sterile screw-capped container. Fungal blood cultures should also be submitted.

Notify Microbiology Department if culture is to r/o Malassezia spp.

Respiratory:

Three separate early morning specimens from a deep cough induced sputum, or specimens collected by bronchoscopy (lavage, washing, brush). transport in sterile container.

Tissues/biopsy:

Collect tissue and transport in sterile container with a small amount of non-bacteriostatic saline to prevent drying.

Do not transport in Formalin

Urine:

First morning clean-catch specimen in a sterile container.

Catheterized specimen

Urine collected in a sterile leakproof container after a prostatic massage.

CSF:

CSF collection tube, 2ml.

Cryptococcal Antigen is performed in lieu of Fungal Stain

Specimen Transport Temperature

Ambient: Deliver within 2 hours.

if delivery is delayed: Refrigerate 2-8°C. 

Specimen Stability Information

24 hours.

Inoculation of appropriate media must occur within 24 hours.

Reject Due To

Tissue specimens received in formalin cannot be cultured.

Swabs are not optimal, but they will be accepted in culture transport media. Dry swabs will be rejected.

CSF:

AFB and fungal culture will only be performed if the cell count is abnormal and either the protein or glucose are abnormal. Specimens that do not meet these criteria will be canceled and the test(s) designated as: "Not Indicated". A report will be generated and the CSF wil be held for 7 days.

Day(s) Performed

Monday through Sunday.

Reference Values

No fungus identified.

Preliminary reports are issued weekly, final report issued after 4 weeks of incubation.

Method Name

Conventional culture and identification method. Mold isolates are referred to Mayo Medical Laboratories (MML) for complete identification.