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Test Code ANDRO Androstenedione, Serum

Reporting Name

Androstenedione, S

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnosis and differential diagnosis of hyperandrogenism, in conjunction with measurements of other sex steroids

 

Diagnosis of congenital adrenal hyperplasia (CAH), in conjunction with measurement of other androgenic precursors, particularly, 17-alpha-hydroxyprogesterone (OHPG), 17 alpha-hydroxypregnenolone, dehydroepiandrosterone sulfate (DHEA-S), and cortisol

 

Monitoring CAH treatment, in conjunction with testosterone, OHPG, DHEA-S, and DHEA

 

Diagnosis of premature adrenarche, in conjunction with measurement of follicle-stimulating hormone and luteinizing hormone as well as other adrenal and gonadal sex-steroids and their precursors


Ordering Guidance


 



Additional Testing Requirements


For diagnosis and differential diagnosis of hyperandrogenism, an initial workup in adults should also include total and bioavailable testosterone (TTBS / Testosterone, Total and Bioavailable, Serum) measurements. Depending on results, this may be supplemented with measurements of sex hormone-binding globulin (SHBG1 / Sex Hormone-Binding Globulin, Serum) and other androgenic steroids (eg, dehydroepiandrosterone sulfate [DHEA-S]).

 

For diagnosis of congenital adrenal hyperplasia (CAH), the following assays should also be ordered:

-OHPG / 17-Hydroxyprogesterone, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-CORT / Cortisol, Serum

 

For monitoring CAH treatment, the following assays should also be ordered:

-TTST / Testosterone, Total, Mass Spectrometry, Serum

-OHPG / 17-Hydroxyprogesterone, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-DHEA_ / Dehydroepiandrosterone [DHEA], Serum.

 

For diagnosis of premature adrenarche, the following assays should also be ordered:

-FSH / Follicle-Stimulating Hormone [FSH], Serum

-LH / Luteinizing Hormone [LH], Serum

-TTBS / Testosterone, Total and Bioavailable, Serum or TGRP / Testosterone, Total and Free, Serum

-EEST / Estradiol, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-DHEA_ / Dehydroepiandrosterone (DHEA), Serum

-SHBG1 / Sex Hormone-Binding Globulin, Serum

-OHPG / 17-Hydroxyprogesterone, Serum



Specimen Required


Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial


Specimen Minimum Volume

0.25 mL

Specimen Type

Serum Red

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  7 days

Day(s) Performed

Monday through Friday

Reference Values

PEDIATRICS*

Premature infants

26-28 weeks, day 4: 92-282 ng/dL

31-35 weeks, day 4: 80-446 ng/dL

Full-term infants

1-7 days: 20-290 ng/dL

1 month-1 year: <69 ng/dL

 

Males*

Tanner stages

Age (Years)

Reference range (ng/dL)

Stage I (prepubertal)

<9.8

<51

Stage II

9.8-14.5

31-65

Stage III

10.7-15.4

50-100

Stage IV

11.8-16.2

48-140

Stage V

12.8-17.3

65-210

 

Females*

Tanner stages

Age (Years)

Reference range (ng/dL)

Stage I (prepubertal)

<9.2

<51

Stage II

9.2-13.7

42-100

Stage III

10.0-14.4

80-190

Stage IV

10.7-15.6

77-225

Stage V

11.8-18.6

80-240

*Soldin SJ, Brugnara C, Wong EC, eds. Androstenedione. In: Pediatric Reference Ranges. 4th ed. AACC Press; 2003: 32-34

 

ADULTS

Males: 40-150 ng/dL

Females: 30-200 ng/dL

 

For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Testing Algorithm

For more information see Steroid Pathways.

Special Instructions

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82157

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANST Androstenedione, S 1854-9

 

Result ID Test Result Name Result LOINC Value
7730 Androstenedione, S 1854-9

Report Available

2 to 5 days