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HelpTest Code AFPAF Alpha-Fetoprotein, Amniotic Fluid
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Alpha Fetoprotein, AFUseful For
Screening for open neural tube defects or other fetal abnormalities
Follow-up testing for patients with elevated serum alpha-fetoprotein results or in conjunction with cytogenetic testing
Special Instructions
Necessary Information
The following information is required:
1. Estimated due date by ultrasound
2. Collection date
3. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.
If not ordering electronically, provide information on Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) and send with specimen.
Specimen Required
Container/Tube: Amniotic fluid container
Specimen Volume: 0.75 mL
Collection Instructions: Do not centrifuge.
Specimen Minimum Volume
0.5 mL
Specimen Type
Amniotic FldSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Amniotic Fld | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days |
Day(s) Performed
Monday through Friday
Reference Values
< 2.0 multiples of median (MoM)
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Method Name
AFPA: Immunoenzymatic Assay
ACHE_: Polyacrylamide Electrophoresis
Testing Algorithm
If alpha-fetoprotein is positive, then acetylcholinesterase will be performed at an additional charge.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ACHE_ | Acetylcholinesterase, AF | Yes | No |
CPT Code Information
82106
82013 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AFPA | Alpha Fetoprotein, AF | 58735-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 6739 | Collection Date | 58734-5 |
| DAT15 | EDD by US Scan | 11781-2 |
| 6740 | Last Menstrual Period (LMP) | 8665-2 |
| 24233 | EDD by LMP | 11779-6 |
| 24239 | GA at Collection by Scan | 11888-5 |
| 24240 | GA at Collection by Dates | 11885-1 |
| 24234 | GA Used | 21299-3 |
| 9950 | Alpha Fetoprotein, AF | 1832-5 |
| 24241 | Results | 29595-6 |
| 24235 | Interpretation | 59462-2 |
| 24236 | Additional Comments | 48767-8 |
| 24237 | Follow up | 80615-8 |
| 24238 | General Test Info | 48767-8 |
Report Available
2 to 19 daysForms
Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) is required.